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March 30, 2017

Articles in Acta Cryst F

Crystal structure of d(CCGGGGTACCCCGG)2 at 1.4 Å resolution

The structure of tetradecamer sequence d(CCGGGGTACCCCGG)2 is reported here as an A-DNA duplex at 1.4 Å resolution.

by Selvam Karthik et al. at March 30, 2017 07:13 AM

Structural insights into the mechanism of the drastic changes in enzymatic activity of cytochrome P450 vitamin D3 hydroxylase (CYP107BR1) caused by a mutation distant from the active site

Structures of the cytochrome P450 citamin D3 hydroxylase inactive mutants, F106V and L348M, were determined at 1.95 Å and 2.7 Å resolution, respectively.

by Yoshiaki Yasutake et al. at March 30, 2017 07:13 AM

Increasing the soluble expression and crystallization of the Escherichia coli quorum-sensing protein LsrK

LsrK from E. coli phosphorylates imported AI-2 molecules and triggers quorum-sensing signalling. The LsrK protein was expressed by applying heat and osmotic shock before IPTG induction, and the crystal of LsrK diffracted to 2.9 Å resolution.

by Ha et al. at March 30, 2017 07:13 AM

X-ray diffraction analysis and in vitro characterization of the UAM2 protein from Oryza sativa

The UAM2 protein from O. sativa was cloned, expressed, purified and crystallized, and a complete data set was obtained from the radiation-sensitive crystals by low-dose vector data collection. In addition, it is shown that UAM2 is likely to exist as a monomer in solution and contains at least one intramolecular disulfide bridge or, alternatively, a structural metal ion.

by Welner et al. at March 30, 2017 07:13 AM

March 29, 2017

Articles in Acta Cryst F

X-ray diffraction analysis and in vitro characterization of the UAM2 protein from Oryza sativa

The role of seemingly non-enzymatic proteins in complexes interconverting UDP-arabinopyranose and UDP-arabinofuranose (UDP-arabinosemutases; UAMs) in the plant cytosol remains unknown. To shed light on their function, crystallographic and functional studies of the seemingly non-enzymatic UAM2 protein from Oryza sativa (OsUAM2) were undertaken. Here, X-ray diffraction data are reported, as well as analysis of the oligomeric state in the crystal and in solution. OsUAM2 crystallizes readily but forms highly radiation-sensitive crystals with limited diffraction power, requiring careful low-dose vector data acquisition. Using size-exclusion chromatography, it is shown that the protein is monomeric in solution. Finally, limited proteolysis was employed to demonstrate DTT-enhanced proteolytic digestion, indicating the existence of at least one intramolecular disulfide bridge or, alternatively, a requirement for a structural metal ion.

by Adams, P.D. at March 29, 2017 12:00 AM

Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals

Superoxide dismutases (SODs) are enzymes that protect against oxidative stress by dismutation of superoxide into oxygen and hydrogen peroxide through cyclic reduction and oxidation of the active-site metal. The complete enzymatic mechanisms of SODs are unknown since data on the positions of hydrogen are limited. Here, methods are presented for large crystal growth and neutron data collection of human manganese SOD (MnSOD) using perdeuteration and the MaNDi beamline at Oak Ridge National Laboratory. The crystal from which the human MnSOD data set was obtained is the crystal with the largest unit-cell edge (240 Å) from which data have been collected via neutron diffraction to sufficient resolution (2.30 Å) where hydrogen positions can be observed.

by Borgstahl, G.E.O. at March 29, 2017 12:00 AM

Nature

An early start on tackling childhood cancers

An early start on tackling childhood cancers

Nature 543, 7647 (2017). doi:10.1038/543590a

The most vulnerable cancer patients are drawing the attention they deserve.

March 29, 2017 12:00 AM

Brexit must preserve advisory networks

Brexit must preserve advisory networks

Nature 543, 7647 (2017). http://www.nature.com/doifinder/10.1038/543591a

Author: James Wilsdon

Policymakers in charge of the United Kingdom’s withdrawal from the European Union have a duty to maintain benefits of collaboration, says James Wilsdon.

by James Wilsdon at March 29, 2017 12:00 AM

Animal behaviour: Playful call makes parrots merry

Animal behaviour: Playful call makes parrots merry

Nature 543, 7647 (2017). doi:10.1038/543592a

Hearing laughter can make people laugh, and it seems that kea parrots react similarly, displaying play behaviour after hearing a particular call from fellow birds. This makes them the first non-mammals known to experience 'contagious' merriment.Raoul Schwing, now at the University of Vienna, and

March 29, 2017 12:00 AM

Planetary science: Titan's electrified dunes

Planetary science: Titan's electrified dunes

Nature 543, 7647 (2017). doi:10.1038/543592b

The dunes of Saturn's largest moon, Titan, may be held together by static electricity.Grains of sand acquire electrostatic charge as they rub against each other, but on Earth this effect is generally negligible because gravity and a high density of heavy silicate particles minimize

March 29, 2017 12:00 AM