Toward the pharmaceutical application of the X-ray free-electron laser, the applicability of ligand soaking method in the serial femtosecond crystallography has been examined.
Structural Biology Planet
June 24, 2017
Articles in Acta Cryst D
Toscana virus nucleoprotein oligomer organization observed in solution
The nucleoprotein of Toscana virus forms mainly open fivefold oligomers consistent with the encapsidation mechanism of the phlebovirus RNA both in solution and under physiological conditions.
Role of conserved surface hydrophobic residues in the carbapenemase activity of the class D β-lactamases
The crystal structure of the class D carbapenemase OXA-143 from A. baumannii shows that a conserved valine residue on the protein surface controls access of the deacylating water molecule to the active site of the enzyme. Analysis of the structures of other class D carbapenemases implicates movement of juxtaposed surface valine and leucine residues in a universal deacylation mechanism for these enzymes.
Activity inhibition and crystal polymorphism induced by active-site metal swapping
The effect of ion swapping on the activity of an Arenaviridae exonuclease is reported.
