Chapter 4: Protein Engineering

Authors:

Sonia Longhi, François Ferron & Marie-Pierre Egloff

Abstract:

This chapter focuses on protein engineering strategies that aim to increase the chances of obtaining crystals suitable for X-ray diffraction. The chapter is divided into three main parts: one dealing with protein engineering through a bioinformatics approach, the second focusing on DNA modifications via random mutagenesis, and the third describing a nonexhaustive number of in vitro modifications based on site-directed mutagenesis.

Pages:

59-90

Details:

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