Structural Biology Planet

Chapter 4: First Analysis of Macromolecular Crystals: Biochemistry and X-Ray Diffraction

Authors:

David Jeruzalmi

Abstract:

Methods are described for performing a first analysis of newly prepared macromolecular crystals. Biochemical analysis using denaturing gel electrophoresis on dissolved crystals informs on the content of the crystal. Detailed protocols are given for mounting crystals in capillaries or fiber loops in preparation for analysis of their X-ray diffractive properties.

Pages:

43-62

Details:

Purchase here